Biosafety Level 2 (BSL-2) Laboratory Compliance Checklist [FREE PDF]

This BSL-2 laboratory compliance checklist verifies containment practices, safety equipment, and training requirements per CDC/NIH BMBL 6th edition and institutional biosafety committee (IBC) requirements. Biosafety officers use this quarterly to ensure labs working with Risk Group 2 agents maintain proper containment and safe practices.

  • Industry: Laboratory
  • Frequency: Quarterly
  • Estimated Time: 30-40 minutes
  • Role: Institutional Biosafety Officer / Lab Manager
  • Total Items: 9
  • Compliance: CDC/NIH BMBL 6th Edition (Biosafety in Microbiological and Biomedical Laboratories), 42 CFR Part 73 (select agents and toxins), IBC (Institutional Biosafety Committee) requirements, OSHA 29 CFR 1910.1030 (bloodborne pathogen standard)

Physical Containment

Verify BSL-2 physical containment requirements.

  • Biological Safety Cabinet(s) certified within past 12 months?
  • All aerosol-generating procedures performed in BSC or equivalent?
  • Autoclave accessible and validated for decontaminating biohazardous waste?
  • Sharps disposed in puncture-resistant, labeled biohazard containers?

PPE & Lab Practices

Verify PPE use and safe laboratory practices.

  • Appropriate PPE (gloves, lab coat, eye protection) available and used?
  • PPE removed before leaving lab and not worn in public areas?
  • No eating, drinking, or applying cosmetics in lab?
  • Handwashing after removing gloves and before leaving lab?
  • BSL-2 inspection notes and deficiencies

Related Laboratory Checklists

Related Biosafety Checklists

Why Use This Biosafety Level 2 (BSL-2) Laboratory Compliance Checklist [FREE PDF]?

This biosafety level 2 (bsl-2) laboratory compliance checklist [free pdf] helps laboratory teams maintain compliance and operational excellence. Designed for institutional biosafety officer / lab manager professionals, this checklist covers 9 critical inspection points across 2 sections. Recommended frequency: quarterly.

Ensures compliance with CDC/NIH BMBL 6th Edition (Biosafety in Microbiological and Biomedical Laboratories), 42 CFR Part 73 (select agents and toxins), IBC (Institutional Biosafety Committee) requirements, OSHA 29 CFR 1910.1030 (bloodborne pathogen standard). Regulatory-aligned for audit readiness and inspection documentation.

Frequently Asked Questions

What does the Biosafety Level 2 (BSL-2) Laboratory Compliance Checklist [FREE PDF] cover?

This checklist covers 9 inspection items across 2 sections: Physical Containment, PPE & Lab Practices. It is designed for laboratory operations and compliance.

How often should this checklist be completed?

This checklist should be completed quarterly. Each completion takes approximately 30-40 minutes.

Who should use this Biosafety Level 2 (BSL-2) Laboratory Compliance Checklist [FREE PDF]?

This checklist is designed for Institutional Biosafety Officer / Lab Manager professionals in the laboratory industry. It can be used for self-assessments, team audits, and regulatory compliance documentation.

Can I download this checklist as a PDF?

Yes, this checklist is available as a free PDF download. You can also use it digitally in the POPProbe mobile app for real-time data capture, photo documentation, and automatic reporting.

Browse More Checklists