Pharmaceutical Water System Inspection Checklist

Pharmaceutical water is a critical utility and raw material for drug manufacturing. This monthly inspection checklist ensures PW and WFI systems meet USP <1231> quality standards and are properly monitored and maintained.

  • Industry: Pharmaceutical & Life Sciences
  • Frequency: Monthly
  • Estimated Time: 30-45 minutes
  • Role: Validation Engineer / QA Manager
  • Total Items: 31
  • Compliance: USP <1231> Water for Pharmaceutical Purposes, FDA 21 CFR 211.67 Equipment Cleaning and Maintenance, EU GMP Chapter 3.41 Water Systems, ISPE Baseline Guide Volume 4 - Water and Steam Systems

Pre-Treatment System

Inspect incoming water pre-treatment.

  • Municipal feed water meeting potable water standards (tested per schedule)?
  • Activated carbon filter replacement current and chlorine removal verified?
  • Water softener or dealkalizer regeneration current?
  • Sediment filters within replacement schedule?
  • UV disinfection lamp intensity monitoring current?

Purification System (RO/EDI/Distillation)',

Inspect water purification equipment.

  • Reverse osmosis (RO) rejection rate within performance specification?
  • EDI or mixed bed DI unit conductivity within specification?
  • WFI still producing WFI within conductivity and endotoxin specs?
  • Water storage tank level and pressure within normal range?
  • Tank vent filter hydrophobic and within replacement schedule?

Distribution Loop

Inspect water distribution system.

  • Water circulating continuously at turbulent flow rate?
  • No newly installed dead legs or unmonitored branches?
  • Loop temperature within specification (hot loops ≥70°C; cold loops ≤25°C)?
  • All points of use included in sampling plan?
  • All loop valves and drains in good condition with no leaks?

Quality Testing and Specifications

Review water quality testing results.

  • Conductivity results within USP specification (PW ≤1.3 µS/cm, WFI ≤1.3 µS/cm)?
  • TOC results within USP specification (≤500 ppb)?
  • Microbial counts within USP and site action limits?
  • WFI endotoxin ≤0.25 EU/mL per USP <85>?
  • No out-of-specification or action limit exceedances in current period?

Sanitization and Maintenance

Verify sanitization program compliance.

  • Sanitization performed per qualified schedule (heat, chemical, or ozone)?
  • Sanitization records complete with parameters and results?
  • Preventive maintenance current for all water system components?
  • In-line conductivity, TOC, and temperature instruments calibrated?
  • Biofilm control strategy documented and effective?

Qualification and Documentation

Verify water system qualification is current.

  • Water system qualification current with Phase I/II/III validation completed?
  • No unqualified changes made to the water system?
  • Monthly water quality trend review completed?
  • Sampling plan current with all critical points included?
  • Water system incident log current?
  • Annual water system review completed per validation protocol?

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Why Use This Pharmaceutical Water System Inspection Checklist?

This pharmaceutical water system inspection checklist helps pharmaceutical & life sciences teams maintain compliance and operational excellence. Designed for validation engineer / qa manager professionals, this checklist covers 31 critical inspection points across 6 sections. Recommended frequency: monthly.

Ensures compliance with USP <1231> Water for Pharmaceutical Purposes, FDA 21 CFR 211.67 Equipment Cleaning and Maintenance, EU GMP Chapter 3.41 Water Systems, ISPE Baseline Guide Volume 4 - Water and Steam Systems. Regulatory-aligned for audit readiness and inspection documentation.

Frequently Asked Questions

What does the Pharmaceutical Water System Inspection Checklist cover?

This checklist covers 31 inspection items across 6 sections: Pre-Treatment System, Purification System (RO/EDI/Distillation)',, Distribution Loop, Quality Testing and Specifications, Sanitization and Maintenance, Qualification and Documentation. It is designed for pharmaceutical & life sciences operations and compliance.

How often should this checklist be completed?

This checklist should be completed monthly. Each completion takes approximately 30-45 minutes.

Who should use this Pharmaceutical Water System Inspection Checklist?

This checklist is designed for Validation Engineer / QA Manager professionals in the pharmaceutical & life sciences industry. It can be used for self-assessments, team audits, and regulatory compliance documentation.

Can I download this checklist as a PDF?

Yes, this checklist is available as a free PDF download. You can also use it digitally in the POPProbe mobile app for real-time data capture, photo documentation, and automatic reporting.

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