Veterinary Diagnostic Laboratory Biosafety Checklist

This comprehensive veterinary diagnostic laboratory biosafety checklist ensures regulatory compliance with USDA APHIS, CDC, OSHA, IATA, BMBL 5th Edition. Biosafety inspection for in-house and referral veterinary diagnostic laboratories covering BSL-2 practices, zoonotic pathogen handling, personal protective equipment, specimen shipping, and USDA/CDC select agent compliance per CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition.. Complete all sections to maintain

  • Industry: Veterinary & Animal Care
  • Frequency: Daily / Per Procedure
  • Estimated Time: 35 minutes
  • Role: Veterinarian / Clinic Manager / Animal Care Lead
  • Total Items: 18
  • Compliance: USDA APHIS, CDC, OSHA, IATA, BMBL 5th Edition

BSL-2 Laboratory Practices

Verify BSL-2 standard practices per BMBL Chapter 3.

  • Is laboratory access restricted to trained personnel with no unauthorized visitors during specimen processing?
  • Are gloves, lab coat, and eye protection worn during all specimen handling per BSL-2 requirements?
  • Are biosafety cabinets (Class II) used for processing potentially infectious specimens generating aerosols?
  • Is mouth pipetting prohibited and mechanical pipetting aids used per BMBL standard practices?
  • Is handwashing conducted after removing gloves and before leaving laboratory per BMBL requirements?
  • Are sharps placed directly in approved sharps containers without recapping or bending?

Zoonotic Pathogen Handling

Verify special precautions for zoonotic agents.

  • Are dermatophyte cultures processed in BSC with appropriate containment to prevent conidia dispersal?
  • Are Brucella cultures and suspect samples handled at BSL-3 level or referred to designated laboratory?
  • Are rabies brain submissions handled per CDC rabies laboratory guidelines with appropriate BSC use?
  • Is laboratory personnel exposure reporting system in place with occupational health evaluation protocol?

Diagnostic Specimen Shipping

Verify IATA and DOT shipping compliance.

  • Are diagnostic specimens shipped as Category B Biological Substance (UN3373) per IATA Packing Instruction 650?
  • Is triple packaging (primary watertight, secondary absorbent, outer rigid) used per P650 requirements?
  • If suspect select agent (FMD, Newcastle Disease), is USDA/CDC emergency notification made before shipping?
  • Have shipping personnel completed IATA DGR dangerous goods shipping training within past 2 years?

Laboratory Quality Management

Verify QM practices per AAVLD accreditation standards.

  • Are current SOPs available for all diagnostic tests performed and within review cycle?
  • Are positive and negative controls included with each diagnostic test run?
  • Is proficiency testing program in place with periodic blind sample evaluation?
  • Is laboratory equipment (centrifuges, incubators, pipettes) calibrated per manufacturer schedule?

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Why Use This Veterinary Diagnostic Laboratory Biosafety Checklist?

This veterinary diagnostic laboratory biosafety checklist helps veterinary & animal care teams maintain compliance and operational excellence. Designed for veterinarian / clinic manager / animal care lead professionals, this checklist covers 18 critical inspection points across 4 sections. Recommended frequency: daily / per procedure.

Ensures compliance with USDA APHIS, CDC, OSHA, IATA, BMBL 5th Edition. Regulatory-aligned for audit readiness and inspection documentation.

Frequently Asked Questions

What does the Veterinary Diagnostic Laboratory Biosafety Checklist cover?

This checklist covers 18 inspection items across 4 sections: BSL-2 Laboratory Practices, Zoonotic Pathogen Handling, Diagnostic Specimen Shipping, Laboratory Quality Management. It is designed for veterinary & animal care operations and compliance.

How often should this checklist be completed?

This checklist should be completed daily / per procedure. Each completion takes approximately 35 minutes.

Who should use this Veterinary Diagnostic Laboratory Biosafety Checklist?

This checklist is designed for Veterinarian / Clinic Manager / Animal Care Lead professionals in the veterinary & animal care industry. It can be used for self-assessments, team audits, and regulatory compliance documentation.

Can I download this checklist as a PDF?

Yes, this checklist is available as a free PDF download. You can also use it digitally in the POPProbe mobile app for real-time data capture, photo documentation, and automatic reporting.

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