Autoclave / Steam Sterilizer Validation Checklist [FREE PDF]
This autoclave validation checklist ensures steam sterilizers used for biohazardous waste decontamination and material sterilization are validated per AAMI ST79, CDC BMBL requirements, and OSHA bloodborne pathogen standard. Biosafety officers and lab managers use this for periodic performance qualification.
- Industry: Laboratory
- Frequency: Quarterly
- Estimated Time: 20-30 minutes
- Role: Biosafety Officer / Lab Manager
- Total Items: 9
- Compliance: AAMI ST79 (comprehensive guide for steam sterilization), CDC BMBL 6th Edition (decontamination requirements), OSHA 29 CFR 1910.1030 (bloodborne pathogens - decontamination), State biohazardous waste regulations
Physical Inspection
Inspect autoclave physical components before validation.
- Door gasket in good condition without cracks or deformation?
- Safety/pressure relief valve functional and last serviced within manufacturer requirement?
- Drain strainer/filter clean and unobstructed?
- Temperature/pressure chart recorder operational and calibrated?
Performance Validation
Conduct performance validation using biological and chemical indicators.
- Chemical integrator placed in worst-case load position passed?
- Geobacillus stearothermophilus biological indicator used and passed (negative growth)?
- Temperature reached 132°C (270°F) for gravity cycle or 121°C (250°F) for minimum?
- Autoclave validation result
- Autoclave validation notes
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Why Use This Autoclave / Steam Sterilizer Validation Checklist [FREE PDF]?
This autoclave / steam sterilizer validation checklist [free pdf] helps laboratory teams maintain compliance and operational excellence. Designed for biosafety officer / lab manager professionals, this checklist covers 9 critical inspection points across 2 sections. Recommended frequency: quarterly.
Ensures compliance with AAMI ST79 (comprehensive guide for steam sterilization), CDC BMBL 6th Edition (decontamination requirements), OSHA 29 CFR 1910.1030 (bloodborne pathogens - decontamination), State biohazardous waste regulations. Regulatory-aligned for audit readiness and inspection documentation.
Frequently Asked Questions
What does the Autoclave / Steam Sterilizer Validation Checklist [FREE PDF] cover?
This checklist covers 9 inspection items across 2 sections: Physical Inspection, Performance Validation. It is designed for laboratory operations and compliance.
How often should this checklist be completed?
This checklist should be completed quarterly. Each completion takes approximately 20-30 minutes.
Who should use this Autoclave / Steam Sterilizer Validation Checklist [FREE PDF]?
This checklist is designed for Biosafety Officer / Lab Manager professionals in the laboratory industry. It can be used for self-assessments, team audits, and regulatory compliance documentation.
Can I download this checklist as a PDF?
Yes, this checklist is available as a free PDF download. You can also use it digitally in the POPProbe mobile app for real-time data capture, photo documentation, and automatic reporting.