Biological Safety Cabinet Inspection and Certification Checklist
This checklist covers NSF/ANSI 49-2019 compliance for Class II Biological Safety Cabinets used in BSL-2 and BSL-3 laboratories. Designed for biosafety officers and laboratory managers responsible for BSC maintenance and certification.
- Industry: Laboratory / Healthcare / Research
- Frequency: Annual certification / Daily operational check
- Estimated Time: 20-30 minutes
- Role: Biosafety Officer / Laboratory Manager
- Total Items: 18
- Compliance: NSF/ANSI 49-2019 Biosafety Cabinetry - Design, Construction, Performance, and Field Certification, CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition, OSHA 29 CFR 1910.1030 Bloodborne Pathogens
Daily Operational Check
Pre-use checks before each laboratory session.
- Airflow alarm and sash alarm functional?
- Inflow airflow indicator within green operational range?
- UV lamp decontamination run before use (where applicable)?
- Sash at correct working height (Class II A2: 8 inches)?
HEPA Filter Performance
HEPA filter condition and performance verification.
- HEPA filter integrity test (DOP/PAO test) passed?
- Supply and exhaust HEPA pressure differentials within limits?
- HEPA filters free of visible damage or bypass?
Airflow Velocity Measurements
Measured inflow and downflow velocity per NSF 49.
- Inflow velocity measured at 75 fpm (+/-10%) per NSF 49?
- Downflow velocity measured at 50-100 fpm as specified?
- Velocity uniformity across work area within specifications?
Physical Condition
Visual inspection of cabinet condition and cleanliness.
- Interior surfaces clean and decontaminated?
- Window gaskets and seals intact?
- Drain valve closed and collection pan clean?
- Blower operating without unusual noise or vibration?
Annual Certification Results
Annual certification test results per NSF/ANSI 49.
- KCl aerosol challenge test passed?
- Certification performed by NSF-certified certifier?
- Certification label affixed with date, result, and next due date?
- Deficiencies or recommendations
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Why Use This Biological Safety Cabinet Inspection and Certification Checklist?
This biological safety cabinet inspection and certification checklist helps laboratory / healthcare / research teams maintain compliance and operational excellence. Designed for biosafety officer / laboratory manager professionals, this checklist covers 18 critical inspection points across 5 sections. Recommended frequency: annual certification / daily operational check.
Ensures compliance with NSF/ANSI 49-2019 Biosafety Cabinetry - Design, Construction, Performance, and Field Certification, CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition, OSHA 29 CFR 1910.1030 Bloodborne Pathogens. Regulatory-aligned for audit readiness and inspection documentation.
Frequently Asked Questions
What does the Biological Safety Cabinet Inspection and Certification Checklist cover?
This checklist covers 18 inspection items across 5 sections: Daily Operational Check, HEPA Filter Performance, Airflow Velocity Measurements, Physical Condition, Annual Certification Results. It is designed for laboratory / healthcare / research operations and compliance.
How often should this checklist be completed?
This checklist should be completed annual certification / daily operational check. Each completion takes approximately 20-30 minutes.
Who should use this Biological Safety Cabinet Inspection and Certification Checklist?
This checklist is designed for Biosafety Officer / Laboratory Manager professionals in the laboratory / healthcare / research industry. It can be used for self-assessments, team audits, and regulatory compliance documentation.
Can I download this checklist as a PDF?
Yes, this checklist is available as a free PDF download. You can also use it digitally in the POPProbe mobile app for real-time data capture, photo documentation, and automatic reporting.