Cannabis Clone and Propagation Room Compliance Checklist

This cannabis clone and propagation room compliance checklist ensures compliance with state cannabis immature plant tracking requirements, sanitation protocols to prevent disease transmission (especially Hop Latent Viroid - HLVd), and GMP standards for genetic material preservation. Contaminated clones are a primary vector for plant disease transmission throughout an entire cultivation facility.

  • Industry: Cannabis
  • Frequency: Weekly
  • Estimated Time: 30-45 minutes
  • Role: Head Grower / Propagation Specialist
  • Total Items: 30
  • Compliance: State Cannabis Immature Plant Tracking Requirements, State Cannabis Clone Transfer and Sale Regulations, GMP Propagation Sanitation Standards, State Cannabis Mother Plant License Requirements, USDA Plant Protection Act Import Restrictions

Mother Plant Management

Mother plant health and tracking.

  • All mother plants tagged per state requirements?
  • Mother plant count matches state tracking system?
  • Mother plants visually healthy — no disease, pest, or nutrient deficiency signs?
  • Mother plants tested for Hop Latent Viroid (HLVd) within last 6 months?
  • Mother plant rotation schedule maintained to keep genetics vigorous?

Clone Cutting Process

Cutting sanitation and technique.

  • Cutting tools sterilized between each mother plant?
  • Sterilization using 70% isopropanol or 10% bleach solution?
  • Gloves changed between mother plants during cutting?
  • Sterile propagation media used for each batch?
  • Rooting hormone products state-approved for use on cannabis?

Propagation Environment

Clone room conditions.

  • Humidity dome or cloner maintaining high RH (80-90%) for rooting?
  • Propagation room temperature maintained at 72-80F (22-27C)?
  • Light schedule at 18/6 hours for vegetative propagation?
  • No powdery mildew or other disease evidence in propagation room?
  • Propagation room physically separate from flowering rooms with controlled access?

Clone Tracking

Immature plant records.

  • Clone batches logged with cutting date, mother plant source, and count?
  • Immature plant counts reported in state tracking system per state rules?
  • Clone losses (deaths, failures to root) recorded in propagation log?
  • Clone movement from propagation to veg room recorded in tracking system?
  • Any clone sales/transfers to other licensees accompanied by state manifest?

Disease Prevention

Propagation biosecurity.

  • New genetic material quarantined and tested before introducing to production?
  • No unverified clones from outside sources without HLVd testing?
  • Propagation room has dedicated tools not shared with other rooms?
  • Foot dip or sanitizing mat at propagation room entrance?
  • Genetic backup plan in place for key strains (tissue culture, preserved cuttings)?

Propagation Records

Documentation.

  • Cutting log current with mother ID, date, count, and media?
  • Clone success rate tracked to identify genetic or process issues?
  • HLVd test results filed for all mother plants tested?
  • Genetic library (strain names, lineage) documented?
  • Propagation Notes

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Why Use This Cannabis Clone and Propagation Room Compliance Checklist?

This cannabis clone and propagation room compliance checklist helps cannabis teams maintain compliance and operational excellence. Designed for head grower / propagation specialist professionals, this checklist covers 30 critical inspection points across 6 sections. Recommended frequency: weekly.

Ensures compliance with State Cannabis Immature Plant Tracking Requirements, State Cannabis Clone Transfer and Sale Regulations, GMP Propagation Sanitation Standards, State Cannabis Mother Plant License Requirements, USDA Plant Protection Act Import Restrictions. Regulatory-aligned for audit readiness and inspection documentation.

Frequently Asked Questions

What does the Cannabis Clone and Propagation Room Compliance Checklist cover?

This checklist covers 30 inspection items across 6 sections: Mother Plant Management, Clone Cutting Process, Propagation Environment, Clone Tracking, Disease Prevention, Propagation Records. It is designed for cannabis operations and compliance.

How often should this checklist be completed?

This checklist should be completed weekly. Each completion takes approximately 30-45 minutes.

Who should use this Cannabis Clone and Propagation Room Compliance Checklist?

This checklist is designed for Head Grower / Propagation Specialist professionals in the cannabis industry. It can be used for self-assessments, team audits, and regulatory compliance documentation.

Can I download this checklist as a PDF?

Yes, this checklist is available as a free PDF download. You can also use it digitally in the POPProbe mobile app for real-time data capture, photo documentation, and automatic reporting.

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